The real-time tracking of a single vesicle containing a quantum dot. Single vesicle moved along axon and fused to plasma membrane. The quenching of fluorescence indicated the fusion of the vesicle and the sudden one-step quenching implied a single quantum dot inside a vesicle.

    Gliding assay of actin filaments by myosin. The (+) end of actin filament was indicated by bright bulbs containing Cy3-labeled the gelsolin .
(a). The gliding assay of actin filaments by myosin V showed that myosin V moved toward the (+) end of actin filaments.
(b). The gliding assay by myosin VI showed that myosin VI moved toward the the (-) end of actin filaments.